Top/fop flash原理
WebArticle Snippet: TOP/FOP-Flash reporter plasmids containing intact or mutated Tcf/Lef binding sites (Addgene, 12,456; Addgene, 12,457) and Renilla plasmids (Promega, E2241) were co-transfected in H1299 cells using Lipofectamine3000 (Invitrogen, USA; 15,282,465). Web用western和免疫荧光验证beta－catenin和下游靶基因的变化只是基础，只是说明你转染了这个抑癌基因的确是改变了catenin通路，但是你想要证明该抑癌基因是通过catenin通路来 …
Top/fop flash原理
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WebLuciferase assays can be used to investigate a variety of cellular processes using bioluminescent light output as a sensitive measure of biological activity. We offer a full range of luciferase assay products and tools for your research. Reporter options include NanoLuc®, firefly and Renilla luciferases, which have different protein sizes ... Web21. feb 2012 · TOPflash is a TCF reporter plasmid containing two sets of three copies of wild-type TCF binding sites driven by the thymidine kinase minimal prompter and …
WebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : Change media with 0 ... Web1. sep 2015 · Top和Fop序列基本上一致的，Top的荧光素酶启动子上有几个Lef/TCF的结合位点，而在FOp是突变的，因此Top/Fop可以检测Wnt的活性改变。 1.应该是要用Top/FOP …
http://www.bioon.com.cn/doc/showarticle.asp?newsid=96420 WebPrice from $9.99 to $1999.99. top fop flash assay - by Bioz Stars , 2024-12. 90 / 100 stars. Buy from Supplier. top fop flash vector ( Promega ) 99. Promega top fop flash vector. Top Fop Flash Vector, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations.
Web10. jan 2024 · For the TOP/FOP-Flash reporter assay, the TOP/FOP-Flash reporter and pTK-RL plasmids were co-transfected into cells. After transfection for 48 h, the cells were harvested for analysis with the ...
WebPlasmid M50 Super 8x TOPFlash from Dr. Randall Moon's lab contains the insert TCF/LEF binding sites and is published in Curr Biol. 2003 Apr 15. 13(8):680-5. This plasmid is … how to cancel in shape gym membershipWebThe Luciferase Assay System incorporates coenzyme A (CoA) for improved kinetics, allowing greater enzymatic turnover and resulting in increased light intensity that is nearly … how to cancel instant ink subscriptionWebTOP or FOP plasmid + Opti-MEM and Lipofectamine + Opti-MEM are incubated at room temp for 5 min respectively. Both solutions are then mixed and incubated for another 15 … mhs certificationWebWe next investigated the effect of irradiation on Wnt/β-catenin pathway activity using TOP/FOP flash luciferase assays and quantitative polymerase chain reaction analysis of β-catenin target genes. The expression levels and activities of two target genes, matrix metalloproteinase (MMP)-2 and MMP-9, were examined further by Western blotting ... how to cancel installation steamWeb29. júl 2024 · TOP/FOP flash assay. The TOP/FOP Flash luciferase assay was performed as previously described . M50 Super 8x TOPFlash and M51 Super 8x FOPFlash (TOPFlash mutant) were gifts from Randall Moon (Addgene, 12,456 and 12,457). PANC-1 human pancreatic cancer cells were transfected with the M50 and M51 plasmids along with an … mhschaefer.comWebTour Start here for a quick overview of the site Help Center Detailed answers to any questions you might have Meta Discuss the workings and policies of this site mhsc handballWebF , The expression of β-catenin by nuclear/cytoplasmic protein fractionation and TOP/FOP-flash reporter assays when silencing MBD2a or overexpressing MBD2c. Data were from 3 independent experiments. ∗ P .05; ∗∗ P .01 by the Student t test. G , The expression of Snail1, OCT4, SOX2 mRNA, and proteins was measured by qPCR and Western blot ... mhsc féminine facebook