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Webb12 jan. 2024 · Thermo Scientific™ SduI (Bsp1286I) (10 U/µL) Corte en sitios GDGCH^C con enzima de restricción SduI (Bsp1286I), que funciona mejor a 37 °C en su propio tampón único (isosquizómeros: Bsp1286I, MhlI). Marca: Thermo Scientific™ ER0651 Detalles … Webb9 apr. 2024 · B16F10 cells (Thermo Scientific, USA). For each experimental group, 2 were cultured in high-glucose DMEM supplemented with replicates of the homogenates were made. Each replicate was 2 mM glutamine, 10% heat-inactivated FBS, 10,000 units/mL made with 3 different cell populations. penicillin, and 10 mg/mL streptomycin. Webb16 nov. 2014 · Then, cells were fixed with 4% methanol-free formaldehyde (Thermo Scientific) for 15 min, permeabilized with 0.1% Triton X-100 for 10 min and blocked with image-iT FX Signal Enhancer (Life Technologies) for 30 min. Vinculin antibody (Sigma) ... 2013; 114 (6):1286–1293. [PMC free article] [Google Scholar] 45. margaret sullivan jewelry new mexico