Pcpf1
Splet03. jun. 2024 · The repair template and the spacer sequence were sequentially cloned into pCpf1 at the sites of BglII/BamHI and AarI/AarI, resulting in the mutation plasmid pCpf1-CT-dnaENI. To generate plasmid pCint2-Δ dnaA , the upstream and downstream sequences of dnaA gene as well as the kanamycin resistance cassette were amplified using specific … SpletpCpf1-sp Sequences (2) Addgene Sequences: Full (1) Depositing Scientist Sequences: Full (1) Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). ...
Pcpf1
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Splet15. apr. 2024 · The results showed that the water flow rate simulated by PCPF1@SWAT2012 was similar with the observed data. The calculated Nash–Sutcliffe efficiency coefficient (NSE) (0.73) and percent bias (PBIAS) (–20.38) suggested satisfactory performance of the model. In addition, the concentrations of herbicides … SpletToxins . 2024, 12, 518; doi:10.3390/toxins12080518 S2 of S6 . M8 T8C (Met 3 Thr) - M9 C253T (R85 stop codon) insertion of TGG203 M10 C253T (R85 stop codon) -
SpletRecommended software programs are sorted by OS platform (Windows, macOS, Linux, iOS, Android etc.) and possible program actions that can be done with the file: like open pf1 … Splet28. maj 2024 · Up to date, to remove plasmids, cells are grown in an antibiotic-free medium for many generations which is time consuming. Recently Niu et al. described the use of the counter selection marker sacB, which encodes the Bacillus subtilis levansucrase for curing the pCpf1 editing vector (a pSL2680-derived plasmid) in Anabaena.
SpletCN107083392A CN202410442623.8A CN202410442623A CN107083392A CN 107083392 A CN107083392 A CN 107083392A CN 202410442623 A CN202410442623 A CN 202410442623A CN 107083392 A CN107083392 A CN 107083392A Authority CN China Prior art keywords sequence crispr cpf1 gene pcr Prior art date 2024-06-13 Legal status … Splet13. mar. 2024 · After KT2440 harboring pCpf1-RK2K, crRNA plasmids were transformed into this strain by electroporation. pSEVA-gR3361T or pSEVA-gR5301T was selected as a control, which was introduced into KT2440 with an equal amount of DNA (150 ng). The related electroporation conditions, genome editing process, and plasmid curing method …
Splet03. apr. 2024 · The PCPF-1@SWAT2012 model is capable of well simulating the water flow rate and transport of herbicides in given watershed, comprising different land use types, …
SpletpCpf1-sp was a gift from Cheng-Cai Zhang (Addgene plasmid # 122186 ; http://n2t.net/addgene:122186 ; RRID:Addgene_122186) For your References section: … the meaning of successionSplet10. feb. 2024 · Cpf1 endonuclease is encoded by pCpf1 vector, crRNA is encoded by pcrRNA vector, target sequence within a split Firefly luciferase gene is encoded by … tiffany scorpion necklace silverSpletMore detailed fieldwork on the PcPF1 and PcPF2 eruptions will allow constraints to be made on the eruptive dynamics of these pre-caldera stage explosive eruptions. Bulk major and trace element geochemistry. Representative samples of the last 12 kyr of activity were analyzed for major and trace elements, ... the meaning of sunglassesSpletProduct code: PCPF1. Planet Fountain Pen Kit Premium Classic Gold 10mm.. € 12.95 Ex Tax: € 10.53. Add to Cart. Add to Wish List. Compare this Product. Add to Wish List. Compare this Product. PLANET PEN TURNING ACCESSORY KIT 10MM. Product code: AK10PP. Planet Pen Turning Accessory Kit 10mm.. ... tiffany sconce light fixturesSplet21. maj 2024 · The isiD deletion plasmid pCpf1-Sp-ΔisiD was constructed similarly except that the homologous repair template was prepared with the primer pairs P0461-up-F/P0461-up-R and P0461-dn-F/P0461-dn-R, and the spacer sequence was annealed with the oligonucleotides cr-0461F109F and cr-0461F109R. The plasmid was transferred into the … tiffany sconces lightingSpletThe results showed that the water flow rate simulated by PCPF1@SWAT2012 was similar with the observed data. The calculated Nash–Sutcliffe efficiency coefficient (NSE) (0.73) and percent bias (PBIAS) (–20.38) suggested satisfactory performance of the model. In addition, the concentrations of herbicides simulated by the PCPF-1@SWAT2012 model ... the meaning of suspiciouslySplet22. apr. 2016 · The result showed that the simulated water flow rate by the PCPF1@SWAT2012 was well predicted with the observed data. The calculated NSE (0.73) and PBIAS (-20.38), suggested the satisfactory ... the meaning of surrogate mother